Journal: Cellular and Molecular Life Sciences: CMLS
Article Title: DDRGK1 preserves intervertebral disc development through ufmylation
doi: 10.1007/s00018-025-06034-8
Figure Lengend Snippet: Ddrgk1 cKO mice showed diminished SOX9 and Aggrecan expression and increased MMP3 expression in intervertebral discs. (A and D) (A) SOX9 IHC staining images in the central region of nucleus pulposus and superior cartilage endplate of WT and Ddrgk1 cKO mice at 12 d, 22 d, 30 d, 6 m, and after LSI surgery. (D) Statistical analyses of SOX9 + cells rate in the nucleus pulposus and cartilage endplate regions between WT and Ddrgk1 cKO mice at the corresponding time points above. n = 6. (B and E) (B) MMP3 IHC staining images in the central region of nucleus pulposus and superior cartilage endplate of WT and Ddrgk1 cKO mice at 12 d, 22 d, 30 d, 6 m, and after LSI surgery. (E) Quantitative analyses of MMP3 expression in the nucleus pulposus and cartilage endplate regions between WT and Ddrgk1 cKO mice at the corresponding time points above. n = 6. (C and F) (C) Aggrecan IHC staining images in the central region of nucleus pulposus and superior cartilage endplate of WT and Ddrgk1 cKO mice at 12 d, 22 d, 30 d, 6 m, and after LSI surgery. (F) Quantitative analyses of Aggrecan expression in the nucleus pulposus and cartilage endplate regions between WT and Ddrgk1 cKO mice at the corresponding time points above. n = 6. Scale bar: 50 μm. Data are represented as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns represents no statistical difference between data
Article Snippet: Aggrecan Polyclonal antibody , Proteintech , Wuhan, Hubei, China , Cat# 13880-1-AP.
Techniques: Expressing, Immunohistochemistry